Constituents of the PCR reaction mixture for each reaction volume of 25 ul per sample for amplification of the HVI, HVII and HVIII regions. PCR cycling conditions for amplification of three hypervariable regions of mitochondrial DNA. Frequency distribution of the mtDNA haplotypes in the Yadav population. Table 6 Sequence polymorphism of the three hypervariable regions and their respective haplogroups in the Yadav population. Table 6 (continued ) Fig. 1. Phylogenetic tree of haplogroups including all related polymorphisms relative to the rCRS for Yadav population. 2.3. Sequencing