High-throughput screening articles from across Nature Portfolio

We developed RNA-linked CRISPR (ReLiC), a high-throughput approach for coupling genetic perturbations to diverse RNA phenotypes. ReLiC enabled the identification of post-transcriptional regulators and uncovered therapeutic targets linked to RNA metabolism.

Aptamers are short, single-stranded DNA or RNA molecules that can bind target molecules with high specificity and affinity and have considerable potential as therapeutic agents and diagnostic tools. A new method, UltraSELEX, identifies optimal sequences from a single round of high-throughput screening.

The human ADAR enzyme can deaminate additional transcripts for therapeutic A-to-G RNA base editing using an antisense guide RNA. Here, authors engineer a universal guide RNA scaffold from the U7 snRNA, boosting editing efficiency from minimal doses, especially in organs with limited AAV delivery.

This protocol describes solid-phase DNA-encoded library synthesis suitable for various high-throughput screening applications. The libraries are constructed using consecutive on-bead chemical synthesis and DNA encoding ligations, resulting in decodable library beads.

CAR T cell manufacturing faces significant challenges that impact cell quality and in vivo efficacy. This necessitates reliable cellular characterization methods. Here the authors present a real-time, label-free, microfluidic method that profiles cellular biophysical properties and correlates them to activation state and CAR T potency, facilitating the rapid phenotypic cell assessment during production.

Human pathways acting on nuclear ncRNAs have been refractory to forward genetics. Here, the authors develop a forward genetic approach that identifies such pathways and show DDX59 is required for minor intron splicing, suggesting a mechanism for its association with Oral-Facial-Digital syndrome.

We developed RNA-linked CRISPR (ReLiC), a high-throughput approach for coupling genetic perturbations to diverse RNA phenotypes. ReLiC enabled the identification of post-transcriptional regulators and uncovered therapeutic targets linked to RNA metabolism.

Aptamers are short, single-stranded DNA or RNA molecules that can bind target molecules with high specificity and affinity and have considerable potential as therapeutic agents and diagnostic tools. A new method, UltraSELEX, identifies optimal sequences from a single round of high-throughput screening.

In this Tools of the Trade article, Abdallah (Gilbert lab) describes the development of direct analysis of ribosome targeting (DART), a tool designed to explore 5' UTR sequences for their potential to efficiently initiate translation.

Multivariate estimators of how much a perturbation affects the overall gene expression phenotype in Perturb-seq data can be derived using univariate statistics from differential gene expression tools.

PROX1 acts as a molecular ‘cell fate guardian’ in hepatocytes, preserving liver cell identity throughout life by actively repressing unwanted plasticity. PROX1 loss impairs liver regeneration and fuels tumor formation, whereas PROX1 activation halts cancer formation and progression, revealing unexpected therapeutic potential.