RNA sequencing articles from across Nature Portfolio

GLORI, a chemical-based quantitative N⁶-methyladenosine (m⁶A) sequencing method, is limited by severe RNA degradation. Two upgraded versions of GLORI have addressed the issue and enable absolute quantification of m⁶A from ultra-low RNA starting material.

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease group with CAR T cells offering therapeutic success in otherwise hard-to-treat cases. Here, authors study the in vivo expansion and persistence of CAR T cells in the peripheral blood of successfully treated DLBCL patients, demonstrating that two different CD8+ precursor phenotypes in the initial cell product give rise to two independent waves of clonally expanded CAR T cells with distinct phenotypes in peripheral blood.

This study employs time-lapse single-nucleus RNA sequencing in Arabidopsis seedlings, revealing distinct circadian rhythms in specific cell types and identifying ABF1 as a regulator that shortens the circadian period.

This work introduces GLORI 2.0 and 3.0, enabling sensitive m⁶A quantification from RNA inputs as low as a few hundred cells.

GLORI, a chemical-based quantitative N⁶-methyladenosine (m⁶A) sequencing method, is limited by severe RNA degradation. Two upgraded versions of GLORI have addressed the issue and enable absolute quantification of m⁶A from ultra-low RNA starting material.

This study generated a transgenic mouse line that enables the in vivo visualization, isolation and transcriptional profiling of oligodendrocyte precursor cells (OPCs) across the lifespan. Analysis of gene-expression changes reveals how OPCs in the cerebral cortex respond to the aging brain environment and highlights pathways that inhibit their differentiation.

Using a combination of single-cell RNA sequencing, T cell and B cell receptor sequencing, and mass cytometry analyses, we characterized the dynamics of peripheral immune cells across the human lifespan. Based on this resource, we present a single-cell immune aging clock to assess an individual’s immune status across different life stages.

Ayako Suzuki recalls two papers published in the early 2000s by the Functional Annotation of the Mammalian Genome (FANTOM) consortium and the Full-Length Long Japan (FLJ) project, which provided reference catalogues of full-length transcripts in mice and humans, respectively.

In this Tools of the Trade article, Ziye Xu and Yongcheng Wang describe snRandom-seq, a high-throughput, high-sensitivity single-nucleus total RNA sequencing technology optimized for archived samples.